Hi JCR, I moved your post to it's own thread. The other thread was quite long and over a year old. Just a note, you should always start your own thread for your project.
The Warm Tiles spacing at 1.5" and 3" alternating was designed to be installed directly to the concrete for heat loss reasons in a direct wire application. If you introduce an insulating layer then you should be installing it at its normal spacing of 3"
There are a few methods that can be done for your type of installation depending on what products may be available to you. Where are you located?
As for the RedGard, it is a crack prevention membrane and does not require the use of the mat for that purpose.
Your heating system is quite large and will require the use of relay kits. Be careful not to go over the maximum amp load (24A)for the relay based on the number of kits needed.
A quick observation based on your square footage looks like you would need three 240V kits to do 400 feet for alternating spacing. That puts you over the max amp rating.
You may have to split up the heat zones to run indepentently of each other.
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Randall